Generation and characterization of an H9N2 cold-adapted reassortant as a vaccine candidate.
Identifieur interne : 001705 ( Main/Exploration ); précédent : 001704; suivant : 001706Generation and characterization of an H9N2 cold-adapted reassortant as a vaccine candidate.
Auteurs : H. Chen [États-Unis] ; Y. Matsuoka ; Q. Chen ; N J Cox ; B R Murphy ; K. SubbaraoSource :
- Avian diseases [ 0005-2086 ] ; 2003.
Descripteurs français
- KwdFr :
- Acclimatation, Animaux, Antigènes viraux (analyse), Basse température, Enfant, Furets, Hong Kong, Humains, RT-PCR, Sous-type H9N2 du virus de la grippe A (immunologie), Sous-type H9N2 du virus de la grippe A (isolement et purification), Sous-type H9N2 du virus de la grippe A (physiologie), Suidae (virologie), Vaccins antigrippaux, Virus recombinants (), Virus recombinants (immunologie), Virus recombinants (isolement et purification), Virus recombinants (physiologie).
- MESH :
- analyse : Antigènes viraux.
- immunologie : Sous-type H9N2 du virus de la grippe A, Virus recombinants.
- isolement et purification : Sous-type H9N2 du virus de la grippe A, Virus recombinants.
- physiologie : Sous-type H9N2 du virus de la grippe A, Virus recombinants.
- virologie : Suidae.
- Acclimatation, Animaux, Basse température, Enfant, Furets, Hong Kong, Humains, RT-PCR, Vaccins antigrippaux, Virus recombinants.
- Wicri :
- geographic : Hong Kong.
English descriptors
- KwdEn :
- Acclimatization, Animals, Antigens, Viral (analysis), Child, Cold Temperature, Ferrets, Hong Kong, Humans, Influenza A Virus, H9N2 Subtype (immunology), Influenza A Virus, H9N2 Subtype (isolation & purification), Influenza A Virus, H9N2 Subtype (physiology), Influenza Vaccines, Reassortant Viruses (classification), Reassortant Viruses (immunology), Reassortant Viruses (isolation & purification), Reassortant Viruses (physiology), Reverse Transcriptase Polymerase Chain Reaction, Swine (virology).
- MESH :
- chemical , analysis : Antigens, Viral.
- geographic : Hong Kong, Influenza Vaccines.
- classification : Reassortant Viruses.
- immunology : Influenza A Virus, H9N2 Subtype, Reassortant Viruses.
- isolation & purification : Influenza A Virus, H9N2 Subtype, Reassortant Viruses.
- physiology : Influenza A Virus, H9N2 Subtype, Reassortant Viruses.
- virology : Swine.
- Acclimatization, Animals, Child, Cold Temperature, Ferrets, Humans, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
H9N2 subtype avian influenza viruses have been identified in avian species worldwide, and infections in pigs were confirmed in Hong Kong in 1998. Subsequently, H9N2 viruses were isolated from two children in Hong Kong in 1999, and five human infections were reported from China, raising the possibility that H9N2 viruses pose a potential pandemic threat for humans. These events prompted us to develop a vaccine candidate to protect humans against this subtype of influenza A viruses. Reassortant H1N1 and H3N2 human influenza A viruses with the six internal gene segments of A/Ann Arbor/6/60 (H2N2)(AA) cold-adapted (ca) virus have been tested extensively in humans and have proved to be attenuated and safe as live virus vaccines. Using classical genetic reassortment, we generated a reassortant that contains the hemagglutinin and neuraminidase genes from A/chicken/Hong Kong/G9/97 (H9N2) and six internal gene segments from the AAca virus. The G9/AAca reassortant virus exhibits the ca phenotype and the temperature-sensitive phenotypes of the AAca virus and was attenuated in mice. The reassortant virus was immunogenic and protected mice from wild-type H9N2 virus challenge. The G9/AAca virus bears the in vitro and in vivo phenotypes specified by the AAca virus and will be evaluated as a potential vaccine candidate in humans.
DOI: 10.1637/0005-2086-47.s3.1127
PubMed: 14575127
Affiliations:
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Le document en format XML
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Chen</name><affiliation wicri:level="1"><nlm:affiliation>Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, G-16, Atlanta, GA 30333, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Influenza Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, G-16, Atlanta, GA 30333</wicri:regionArea><wicri:noRegion>GA 30333</wicri:noRegion></affiliation></author><author><name sortKey="Matsuoka, Y" sort="Matsuoka, Y" uniqKey="Matsuoka Y" first="Y" last="Matsuoka">Y. Matsuoka</name></author><author><name sortKey="Chen, Q" sort="Chen, Q" uniqKey="Chen Q" first="Q" last="Chen">Q. Chen</name></author><author><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name></author><author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name></author><author><name sortKey="Subbarao, K" sort="Subbarao, K" uniqKey="Subbarao K" first="K" last="Subbarao">K. Subbarao</name></author></analytic><series><title level="j">Avian diseases</title><idno type="ISSN">0005-2086</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acclimatization</term><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Child</term><term>Cold Temperature</term><term>Ferrets</term><term>Hong Kong</term><term>Humans</term><term>Influenza A Virus, H9N2 Subtype (immunology)</term><term>Influenza A Virus, H9N2 Subtype (isolation & purification)</term><term>Influenza A Virus, H9N2 Subtype (physiology)</term><term>Influenza Vaccines</term><term>Reassortant Viruses (classification)</term><term>Reassortant Viruses (immunology)</term><term>Reassortant Viruses (isolation & purification)</term><term>Reassortant Viruses (physiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Swine (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acclimatation</term><term>Animaux</term><term>Antigènes viraux (analyse)</term><term>Basse température</term><term>Enfant</term><term>Furets</term><term>Hong Kong</term><term>Humains</term><term>RT-PCR</term><term>Sous-type H9N2 du virus de la grippe A (immunologie)</term><term>Sous-type H9N2 du virus de la grippe A (isolement et purification)</term><term>Sous-type H9N2 du virus de la grippe A (physiologie)</term><term>Suidae (virologie)</term><term>Vaccins antigrippaux</term><term>Virus recombinants ()</term><term>Virus recombinants (immunologie)</term><term>Virus recombinants (isolement et purification)</term><term>Virus recombinants (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Hong Kong</term><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sous-type H9N2 du virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H9N2 Subtype</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H9N2 Subtype</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H9N2 du virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H9N2 du virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H9N2 Subtype</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Suidae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acclimatization</term><term>Animals</term><term>Child</term><term>Cold Temperature</term><term>Ferrets</term><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acclimatation</term><term>Animaux</term><term>Basse température</term><term>Enfant</term><term>Furets</term><term>Hong Kong</term><term>Humains</term><term>RT-PCR</term><term>Vaccins antigrippaux</term><term>Virus recombinants</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Hong Kong</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H9N2 subtype avian influenza viruses have been identified in avian species worldwide, and infections in pigs were confirmed in Hong Kong in 1998. Subsequently, H9N2 viruses were isolated from two children in Hong Kong in 1999, and five human infections were reported from China, raising the possibility that H9N2 viruses pose a potential pandemic threat for humans. These events prompted us to develop a vaccine candidate to protect humans against this subtype of influenza A viruses. Reassortant H1N1 and H3N2 human influenza A viruses with the six internal gene segments of A/Ann Arbor/6/60 (H2N2)(AA) cold-adapted (ca) virus have been tested extensively in humans and have proved to be attenuated and safe as live virus vaccines. Using classical genetic reassortment, we generated a reassortant that contains the hemagglutinin and neuraminidase genes from A/chicken/Hong Kong/G9/97 (H9N2) and six internal gene segments from the AAca virus. The G9/AAca reassortant virus exhibits the ca phenotype and the temperature-sensitive phenotypes of the AAca virus and was attenuated in mice. The reassortant virus was immunogenic and protected mice from wild-type H9N2 virus challenge. The G9/AAca virus bears the in vitro and in vivo phenotypes specified by the AAca virus and will be evaluated as a potential vaccine candidate in humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Chen, Q" sort="Chen, Q" uniqKey="Chen Q" first="Q" last="Chen">Q. Chen</name><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name><name sortKey="Matsuoka, Y" sort="Matsuoka, Y" uniqKey="Matsuoka Y" first="Y" last="Matsuoka">Y. Matsuoka</name><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name><name sortKey="Subbarao, K" sort="Subbarao, K" uniqKey="Subbarao K" first="K" last="Subbarao">K. Subbarao</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Chen, H" sort="Chen, H" uniqKey="Chen H" first="H" last="Chen">H. 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